Purification, Properties, and Feedback Control of l-Threonine Dehydratase from Spinach
نویسندگان
چکیده
منابع مشابه
Isolation and Properties of a Homogeneous Preparation of Cystathionine Synthetase-l-serine and L-threonine Dehydratase.
Selim and Greenberg (1, 2) achieved a considerable degree of purification of L-serine dehydratase (L-serine hydro-lyase (deaminating), EC 4.2.1.13) from rat liver and demonstrated that this protein preparation contained the cystathionine-synthesizing activity of the liver (L-serine hydro-lyase (adding L-homocysteine), EC 4.2.1.21). These workers (2) also observed activity of their enzyme prepar...
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Materials-L-Threonine, L-serine, and pyridosal-P monohydrate were purchased from the California Corporation for Biochemical Research, a-ketobutyric acid from the Nutritional Biochemical Corporation, pyruvic acid from the Fisher Scientific Company, and DESE-cellulose from Eastman Organic Chemicals. nn-Allothreonine and L-allothreonine were kindly furnished by Dr. Alton iLleister of Tufts Univers...
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The ADP-activated threonine dehydratase of Closfridium fefanomorphum has been purified approximately llOO-fold in 20% yield and crystallized. Such preparations are homogeneous when examined by sedimentation, electrophoretic, and immunological techniques. The pure enzyme displays an absorption maximum at 415 rnp in neutral solutions, which is consistent with a Schiff base linkage between pyridox...
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The chloroplastic glutamine synthetase of spinach leaves has been purified to homogeneity using affinity chromatography. This involves a tandem ;reactive blue A-agarose' and ;reactive red-A-agarose' as the final step in the procedure. This procedure results in a yield of 18 milligrams of pure glutamine synthetase per kilogram of starting material. The purity of our enzyme has been demonstrated ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63089-3